■ 基本信息
別名: | pETcoco1 |
啟動(dòng)子: | T7 |
原核抗性: | Chl |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 大腸桿菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Chl |
真核抗性: |
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡(jiǎn)介
The pETcoco expression vectors are designed to allow “on command” amplification of vector copy number from single copy (1–2 plasmid copies per cell) to medium copy (20– 50 per cell). The vector is maintained in the single copy state by utilizing the oriS and repE elements of the F episome together with the parABC partition determinants. The medium copy state is obtained by employing the RK-2 derived trfA replicator acting at oriV. The switch from single copy to medium copy status is achieved by inducing the expression of the trfA gene with arabinose. The dual replicon nature of these vectors promotes enhanced stability of recombinant plasmids and extremely low basal expression levels in the single copy state, while allowing robust target protein expression typical of the pET vectors. Unique restriction sites are shown on the circle map (below). The sequence is numbered relative to the start of T7 transcription at +1.。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK1769 pETcoco-1大腸表達(dá)質(zhì)粒.txt
