■ 基本信息
啟動(dòng)子: | P43 |
質(zhì)粒分類: | 廣宿主系列，枯草桿菌載體 |
質(zhì)粒大小: | 3787bp |
原核抗性: | Kan |
克隆菌株: | BS168 |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 枯草芽孢桿菌 |
| 5'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 枯草桿菌 |
質(zhì)粒用途: | 蛋白表達(dá),分泌表達(dá) |
片段類型: | ORF |
片段物種: | 空載體
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原核抗性: | Kan |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
pWB705 andpWB980 . pUB110 is the parental plasmid for pUB18 and its derivatives.pWB705 is a pUB18-based secretion vector carrying both the constitutive expressed P43 promoter and the engineered levansucrase signal sequence. pWB980 is a pUB19-based secretion vector. The transcription direction of the P43 promoter is the same as that for the majority of the pUB110-encoded genes. rep, kan, ble, mob and sacB SP represent thesequence coding for replicase, kanamycin resistance marker,bleomycin resistance marker, mobilization protein and the levansucrase signal sequence, respectively. The arrows show the transcription directions for these genes.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列可參考（具體以測(cè)序?yàn)闇?zhǔn)）下載：
ZK1024pWB980枯草分泌質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
