■ 基本信息
啟動子: | CMV |
復制子: | pUC |
質粒大小: | 7567bp |
原核抗性: | Amp |
真核抗性: | EGFP |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
■ 質粒屬性
質粒宿主: | 哺乳細胞,慢病毒 |
質粒用途: | 基因沉默 |
片段類型: | shRNA
|
片段物種: |
|
原核抗性: | Amp |
真核抗性: |
|
熒光標記: | 綠色
|
■ 質粒簡介
EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
（pSico shRNA cloning protocol點擊下載）
pSicoshRNAcloningprotocol.pdf
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK826pSicoR慢病毒干擾質粒.txt
